5-Aminofluorescein and 6-aminofluorescein are commonly used in elucidation of protein and membrane structures, analysis of nucleic acids and nucleotides, lipid transport and metabolism due to the convenient coupling of the amino group with an activated carboxylic group of the target molecules. [1]
Fluorophores
A fluorophore (or fluorochrome) is a fluorescent chemical compound that can re-emit light upon light excitation. Fluorophores typically contain several combined aromatic groups, or planar or cyclic molecules with several π bonds.[2]
- Green is a Fluorescein
- Red is Rhodamine B
- Yellow is Rhodamine 6G
- Blue is Quinine
- Purple is a mixture of Quinine and Rhodamine 6g
Fluorescein probes application
The fluorescent probes play important roles in the life and environmental sciences. Having met various chemical and biological property requirements, fluorescein-based probes (our 5-Aminofluorescein and 6-Aminofluorescein ) as a common class of fluorescent reagents have proven to be a sensitive, specific analytical tool in life sciences and chemical analyses as well as replacement of radioisotope uses.
References
[1] Mini-Reviews in Organic Chemistry, 2009, 6, 35-43
[2] Fluorescence Microscopy in Life Sciences. Bentham Science Publishers. pp. 61–95
[3] Molecules2012, 17(12), 14067-14090
